De-Solv-it has been written up as safe in the American Journal of Surgery and Emergency Medicine Journal. It has been used on delicate neo-natal skin to prevent skin tearing when removing adhesive from skin. De-Solv-it was also used on over 12,000 workers at the Alaskan Oil Spill to successfully remove the oil and mousse from skin and hair. Numerous hospitals and medical groups have recognized De-Solv-it for its safety and performance saving lives.
Safety Test Overview - De-Solv-it
The following summaries demonstrate the safety characteristics of De-Solv-it. These summaries were conducted in vivo on small mammals under controlled laboratory conditions. Actual experience in hospitals across the United States has reaffirmed the safety of De-Solv-it.
MUSCLE TISSUE TEST
Procedures: Three male New Zealand rabbits weighing between 2-3 kg. were used for this study. The backs of the animals were clipped free of fur and they were anesthetized. Four incisions were made into the para-vertebral muscle of each rabbit. The incision were 3 cm. long and 4-5 mm. deep. Two of the incisions served as controls. One of the remaining incisions was wiped with surgical cotton containing De-Solv-it for two minutes. Into the remaining incision, one ml. of De-Solv-it was introduced for five minutes before being wiped dry. The animals were observed for 48 hours, sacrificed, and treated tissue surgically removed and preserved. The tissues were then examined histopathologically.
Results: Gross examination of the tissue showed no visual signs of deterioration.
CONCLUSION
There is a tendency to induce a moderate to severe acute inflammatory reaction,
with tissue necrosis. There is some variation to the degree of inflammation,
which may be a reflection of the time or dose of exposure.
INHALATION TOXICITY
Procedure: Ten male Sprague-Dawley rats weighing between 200-300 grams were used for this study. The animals were housed in individual compartments in an inhalation chamber. A 15 minute equilibration period was allowed. The animals were observed during exposure and for the following 14 days.
Results: There were no abnormal behavioral or physical characteristics observed. Average weight gain over the 2 week period was 83 grams. Average weight gain for 4 control rats was 52 grams. There were no deaths. At the end of the observation period, the animals were sacrificed and autopsied. All animals appeared normal. A total of 60 ml. of the test sample was vaporized during the exposure period. Chamber concentrations were 1846 ppm at 30 minutes and 1249 ppm at 60 minutes.
CONCLUSION
There were no toxic manifestations under the conditions of this study.
DRAIZE EYE IRRITATION
Procedures: Nine adult albino rabbits with normal eyes were used to evaluate De-Solv-itŪ for degree of eye irritation. 0.1 ml. of product was instilled in one eye of each rabbit. The other untreated eye served as a control. In six of the animals, the product was instilled and the animal released. In the remaining three, each test eye was immediately washed with at least 20 ml. of lukewarm water. Ocular readings were recorded at 24, 48, and 72 ours after instillation. Each eye was scored for the cornea, iris, and conjunctiva. A composite score was then calculated.
Results: For the purpose of evaluating eye irritation, only the six animals in which eyes were not washed are considered relevant. No corneal opacity or redness of the conjunctiva were observed at 24, 48, or 72 hour readings. The iris also appeared normal at each scoring session.
CONCLUSION
De-Solv-it meets the requirements of the Consumer Product Safety Commission
for classification as a non-irritant to the eye.
ORAL TOXICITY TEST
Procedure: Sprague-Dawley rats weighing between 200-300 grams were selected for this study. Ten males were treated at a dosage level of 16 ml./kg. The animals were housed as a group in a temperature controlled room (70o F). Food was withheld for 24 hours prior to intubation of the test sample. Water was allowed ad libitum. Following the oral administration of the test sample, food and water were allowed ad libitum. The test animals were observed for 14 days.
Results: There were no abnormal behavioral or physical characteristics noted during the observation period. No test animals died during the 14 days following intubation. Average weight gain at the end of the observation period was 38 grams.
CONCLUSION
De-Solv-it was non-toxic to rats at a dosage level of 16 ml./kg. It can
be considered practically non-toxic. The LD50 would be in excess of 16 ml./kg. A 150 lb. human (70 kg.) could ingest 1120 grams (2.47 lbs.) without
suffering death or physiological injury.
PRIMARY SKIN IRRITATION
Procedure: Six adult New Zealand albino rabbits were used to evaluate the degree of skin irritation of De-Solv-itŪ. The trunk of each animal was clipped free of hair and two areas on the back about 10 cm. apart were designated for patch placement. On one of the sites, minor incisions through the stratum corneum were made, but not sufficiently deep to disturb the derma or produce bleeding. The patches consisted of a square of clear plastic sandwiched between two layers of gauze. The plastic retards evaporation during the 24 hour exposure. 0.5 ml. of test liquid was introduced beneath each patch, which was then secured with adhesive tape. Evaluations of both intact and abraded skin were made for erythema and eschar formation and presence of edema at 24 and 72 hours.
Results: Slight edema was observed in one rabbit at 24 hours on both the intact and abraded skin. The irritation index was computed to be 0.2.
CONCLUSION
De-Solv-it was found to be a non-irritant
to skin under Consumer Product
Safety Commission Standards.
Note: All tests were independently done by Applied Biological Sciences Laboratory, Glendale, CA.